This study aims to determine the effect of germination time on the antioxidant activity of Leucaena leucocephala (lmk.) de Wit (lead tree) sprouts. Determination of antioxidant activity was carried out on ethanol extract of germinated seeds using β-carotene bleaching assay. The results showed that the antioxidant activity of germinated seeds were affected by germination time. Germination for 4 days affected the greatest enhancement in antioxidant activity by 2.78 fold and caused a significant increment in total phenolic content by 7.29, β-carotene by 3.27, ascorbic acid by 3.81 and α-tocopherol by 4.58 fold, respect to imbibed seeds. The total phenolics content was 53.42±0.22 mg CAE/g dw, β-carotene was 530.99±71.13 10-3 mg/100 g, ascorbic acid was 152.37±2.06 mg/100 g, and α-tocopherol was 59.27±0.10 mg/100 g sample. The findings suggested that the L. leucocephala sprouts could be considered as a source of natural antioxidant.