View Article |
Commercial lactic acid bacteria and probiotic strains- tolerance to bile, pepsin and antibiotics
Ashraf, R1, Smith, S.C2.
Screening and characterization of probiotic strains is crucial for achieving expected health
benefits. In the current study, seventeen lactic acid bacteria (LAB) and probiotic strains were
screened for survival in simulated gastric juice (pH 3 and 2) and bile (0.5% or 2.0%) for 3 and
12h, and antibiotic tolerance pattern using Etest® and Kirby Bauer Disc diffusion method. All
tested strains exhibited survival during simulated gastric transit at pH 3 for 3 h. Lactobacillus
reuteri, L. rhamnosus G5435, L. acidophilus 388, L. delbrueckii subsp. bulgaricus 11842,
Streptococcus thermophilus 1342, Bifidobacterium lactis BB12 and S. thermophilus M5
were found intrinsically tolerant to gastric and small intestinal transit and most tolerant
strains among tested LAB (% survival ≥ 55). All strains were susceptible to ampicillin and
erythromycin. Vancomycin and streptomycin tolerances were most common among species
whereas tolerances for gentamicin, clindamycin and tetracycline were rare. The tolerances
could provide additional benefit to strains in colonizing and replenishing gut microbiota after
antibiotic therapy. The results obtained in the study confirm that strain viability in gastric and
bile solution and antibiotic susceptibility are important attributes in the selection of potentially
probiotic bacteria.
Affiliation:
- Victoria University, Australia
- Deakin University, Australia
|
|
Indexation |
Indexed by |
MyJurnal (2018) |
H-Index
|
8 |
Immediacy Index
|
0.000 |
Rank |
28/30,Q4(Sciences ) 28/30,Q4(Sciences ) 14/17,Q4(Medical & Health Sciences )
|
Indexed by |
Scopus (SCImago Journal Rankings 2016) |
Impact Factor
|
- |
Rank |
Q3 (Food Science) |
Additional Information |
0.335 (SJR) |
|
|
|