The preservation of quality, health and nutritional value of the seeds during the storage period depends not only on the conditions of production and harvesting, but the storage and maintenance of appropriate storage conditions of the product. Thus, the aim of this study was to analyze the chemical composition of seeds of landraces maize stored under different conditions. Seeds of three maize varieties Oito Carreiras, Cabo Roxo and Lombo Baio were analyzed in relation to the contents of ash, crude protein, lipids, fatty acids, total dietary fiber, soluble and insoluble, and carbohydrates. From the results it was concluded that the content of crude protein, fat and saturated fatty acids decreased with storage and that the chemical composition of the seeds of landraces of maize did not vary with the storage condition. The chemical composition of stored seeds is modified in intensity and variable speed as it progresses deterioration in reserve substances, respiratory rate, synthesis and activity of enzymes can occur, all with direct influence on the chemical composition of the seeds.