In recent years, there is a tremendous proliferation and growth in the development of mobile applications. An important aspect in the advancement of this technology is the usability evaluation of these mobile applications. In this study, a laboratory-based mobile usability assessment was conducted on the Amazon Kindle e-book reader app using 15 users who performed five tasks on the mobile app. A post-test questionnaire was administered to capture users’ perception of the mobile usability of the app. The outcomes show that most of the participants were satisfied and delighted with the services offered by the app. On all the four user experience factors evaluated, that is, perceived ease-of-use, perceived visibility, perceived enjoyability, and perceived efficiency, the outcome shows that the participants had an excellent mobile experience with the app.