This study aimed to determine the best parameters (types of buffer, hydrolysis time and enzyme concentration) used to produce good quality of liquid protein hydrolysate from Yellowstripe scad in terms of high yield, protein content and concentration. The choice of buffer (sodium or potassium buffer), hydrolysis time (1 h, 2 h, 3 h or 4 h) and enzymes concentrations (0.5%, 1.0%, 1.5% and 2.0%) were investigated. The results obtained from two way ANOVA showed that these parameters had significant difference (p<0.05) indicating these three parameters had interaction among each other. The optimum conditions found were sodium buffer with 1h and 2 h of hydrolysis time, in terms of have higher percentage of yield (70 – 80%), protein content (4.2 – 5.8%) and concentration of protein hydrolysate (68 – 92 mg/ml).