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Selective recovery of amylase from pitaya (Hylocereus polyrhizus) peel using alcohol/salt aqueous two-phase system
Chow, Yin Hui1, Ow, Jun Leong2.
Amylase, which can hydrolyze starch into oligosaccharides, is a significant
enzyme with diverse applications in various industries. In comparison to
microbial amylases, plants can serve as a rich source of plant-derived amylase
for industrial production at lower cost and toxicity. In this study, the
ethanol/phosphate aqueous two phase system (ATPS) was adopted to recover the
amylase from the red pitaya peel. The effects of several ATPS parameters such
as concentrations of ethanol and phosphate, the system pH, and the concentration
of sodium chloride (NaCl) on the recovery of amylase were investigated. Based
on these results, a central composite design (CCD) was used to optimize the
recovery of amylase from the red pitaya peel. Results demonstrated that the
optimal conditions for recovery of amylase were observed for the ATPS
containing 19% (w/w) ethanol, 26% (w/w) phosphate pH 7.0, and 3.8% (w/w)
NaCl. The amylases were recovered to the ethanol-rich top phase with an
optimum yield and a purification factor of 92.48% and 5.75, respectively.
Therefore, this study demonstrated the feasibility of ethanol/phosphate ATPS as
a promising tool for the simultaneous recovery and purification of amylase from
the red pitaya peel.
Affiliation:
- Taylor's University, Malaysia
- Taylor's University, Malaysia
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Indexation |
Indexed by |
MyJurnal (2019) |
H-Index
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0 |
Immediacy Index
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0.000 |
Rank |
0 |
Indexed by |
Scopus (SCImago Journal Rankings 2016) |
Impact Factor
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- |
Rank |
Q3 (Engineering (miscellaneous)) |
Additional Information |
0.193 (SJR) |
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