Sheep hooves were subjected to an enzymatic hydrolysis using papain to produce hooves hydrolyzate (HH). Some HH based formulations were tested to support Lactobacillus farciminis DSM20184 growth, as a result, the combination of HH and 20 g L-1 of glucose was selected as Hooves Medium (HM) and compared with MRS to cultivate other lactic acid bacteria. Generally, HM showed a good performance to support the strains growth, L. fermentum DSM20049 and L. farciminis showed the most significant results with maximal OD600 that are respectively 67.24% and 33.77% higher than those obtained on the standard medium MRS. Hence, the formulation of HM could constitute a good way to exploit sheep hooves and may be proposed as a suitable solution to reduce LAB biomass production costs.