View Article |
Detection of Listeria monocytogenes in foods using monoclonal antibody by dot-ELISA and multiplex-PCR
Prommajan, K1, Palaga, T2, Rengpipat, S3.
Monoclonal antibodies (MAbs) against Listeria monocytogenes were raised using the NCTC
11994 and EGD-e isolates of L. monocytogenes to immunize BALB/c mice. The antigenic
protein of 65 kDa from L. monocytogenes EGD-e was found to react with MAb LMF3-238.
This MAb characterized as IgG1 specifically reacted with 11 out of 12 different strains of L.
monocytogenes, covering serotypes 1/2a, 1/2b, 1/2c, 4a and 4b that are virulent markers. MAb
LMF3-238 detected L. monocytogenes EGD-e after inoculation into pork meat and storage at
4°C for 36 hours at a limit of detection of ~106 colony forming units/ml by dot-ELISA. From
311 Listeria spp. colonies, isolated from a total of 50 different fresh and processed foods and
randomly selected from PALCAM agar after enrichment, L. monocytogenes was identified
by MAb LMF3-238 using dot-ELISA with relative accuracy, relative sensitivity and relative
specificity of 95.2%, 92.5% and 98.0%, respectively, compared to the multiplex-PCR method.
Affiliation:
- Chulalongkorn University, Thailand
- Chulalongkorn University, Thailand
- Chulalongkorn University, Thailand
|
|
Indexation |
Indexed by |
MyJurnal (2017) |
H-Index
|
8 |
Immediacy Index
|
0.002 |
Rank |
24/42,Q3(Sciences ) 24/42,Q3(Sciences ) 8/20,Q2(Medical & Health Sciences )
|
Indexed by |
Scopus (SCImago Journal Rankings 2016) |
Impact Factor
|
- |
Rank |
Q3 (Food Science) |
Additional Information |
0.335 (SJR) |
|
|
|