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Lactic acid production from free and Polyurethane immobilized cells of Rhizopus oryzae MTCC 8784 by direct hydrolysis of starch and agro-industrial waste
Ranjit, C1, Srividya, S2.
This study investigated the production of L-lactic acid (LA) from agro-industrial waste by
free and polyurethane immobilized Rhizopus oryzae MTCC 8784 using the innate hydrolytic
potential of the fungus. Fungal biomass and lactic acid production obtained from the agrowastes
(from all 3 pre-treatments) were compared to the yield parameters obtained with starch.
Sugar bagasse on acid hydrolysis gave the highest lactic acid yield of 79.2 g/L, with highest
volumetric productivity of 1.1 g/L/h. Cultivation of polyurethane (PU) sponge immobilized
R. oryzae MTCC 8784 on starch utilization at 30°C, 160 rpm of agitation rate and pH of 6.0
for 72 h, showed 1.15 fold increase in LA concentration in comparison to free cells. Lactic
acid was successfully obtained from amylolytically saccharified starch by 4 repeated batch
cultures with stable efficiency. All the agrowaste substrates tested supported higher lactic acid
yield as compared to free cells using PU immobilized R.oryzae MTCC 8784 at 30°C, 160 rpm
of agitation and pH of 6.0 for 72 h with sugar bagasse showing a maximum yield of 88.2g/L
(1.22 fold higher than with free cells). HPLC chromatogram revealed lactic acid peak with a
retention time of 2.61 min. To our knowledge, this is the first report on lactic acid production by
innate hydrolysis of agrowastes under immobilized condition by a fungus. The isolate MTCC
8784 can be a good candidate for cost-effective and ecofriendly industrial production of lactic
acid from agro-industry waste.
Affiliation:
- Jain University, India
- Jain University, India
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Indexation |
Indexed by |
MyJurnal (2019) |
H-Index
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0 |
Immediacy Index
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0.000 |
Rank |
0 |
Indexed by |
Scopus (SCImago Journal Rankings 2016) |
Impact Factor
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- |
Rank |
Q3 (Food Science) |
Additional Information |
0.335 (SJR) |
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